tdTomato Antikörper

Produktnummer ABIN7273105

Der Ziege Polyklonal anti-tdTomato Antikörper (ABIN7273105) detektiert spezifisch tdTomato in IF, IHC (fro), WB, IEM und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Discosoma und Tomato.

Kurzübersicht für tdTomato Antikörper (ABIN7273105)

Target: tdTomato (tdTomato Fluorescent Protein (tdTomato))

Reaktivität: Discosoma, Tomato

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser tdTomato Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Immunoelectron Microscopy (IEM), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-tdTomato Antikörper

Verwendungszweck: anti-tdTomato

Sequenz: MVSKGEEDNM AIIKEFMRFK VHMEGSVNGH EFEIEGEGEG RPYEGTQTAK LKVTKGGPLP FAWDILSPQF MYGSKAYVKH PADIPDYLKL SFPEGFKWER VMNFEDGGVV TVTQDSSLQD GEFIYKVKLR GTNFPSDGPV MQKKTMGWEA SSERMYPEDG ALKGEIKQRL KLKDGGHYDA EVKTTYKAKK PVQLPGAYNV NIKLDITSHN EDYTIVEQYE RAEGRHSTGG MDELYK

Spezifität: In 293HEK cells transfected with cds plasmid detects a band of 54 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent protein).

Kreuzreaktivität (Details): Reacts against tdTomato, mCherry, RFP

Produktmerkmale: Red Fluorescent Protein

Aufreinigung: Epitope affinity purified

Immunogen: Purified recombinant peptide produced in E. coli.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 2 mg/mL

Buffer: preservatives

Handhabung: The antibody solution should be gently mixed before use.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

Details zu tdTomato

Target: tdTomato (tdTomato Fluorescent Protein (tdTomato))

Andere Bezeichnung: tdTomato

Hintergrund:

Goat polyclonal antibody to tdTomato (red fluorescent protein). tdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation. It is a ~54 kDa protein that is optimally excited at a 554 nm and has a maximum of emission at 581 nm. It´s brightness and emission wavelength, makes it ideal for live animal research.

red fluorescent protein antibody.

Molekulargewicht: 54 kDa