mGrape Fluorescent Protein Antikörper

Produktnummer ABIN7273104

Der Ziege Polyklonal anti-mGrape Fluorescent Protein Antikörper wird verwendet zum Nachweis von mGrape Fluorescent Protein in Proben von Discosoma. Er wurde validiert für IEM, IF, IHC (fro), IHC (p) und WB.

Kurzübersicht für mGrape Fluorescent Protein Antikörper (ABIN7273104)

Target: mGrape Fluorescent Protein

Reaktivität: Discosoma

Wirt: Ziege

Klonalität: Polyklonal

Applikation: Immunoelectron Microscopy (IEM), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Produktdetails

Kreuzreaktivität (Details): Reacts against Red Fluorescent Protein

Aufreinigung: Epitope affinity purified

Immunogen: Purified recombinant fluorescent protein produced in E. coli.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500

Kommentare:

In 293HEK cells transfected with cds plasmid detects a band of 25.4 kDa by Western blot. This antibody does not recognize GFP (green fluorescent protein).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 3 mg/mL

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20 °C for long-term storage. Store at 2-8 °C for up to one month.

Antigendetails

Target: mGrape Fluorescent Protein

Hintergrund: MGrape (mGrape fluorescent protein) is a basic (constitutively fluorescent), monomeric engineered derivate of red fluorescent protein (RFP) isolated from members of the Discosoma (mushroom coral) family. mGrape is a ~25.4 kDa protein that is optimally excited at a 595 nm and has a maximum of emission at 625 nm. It is reported to be a somewhat slowly-maturing monomer with low acid sensitivity. It is used in research as a reporter to label and study the biology of the cell using a wide range of applications.