mApple Fluorescent Protein Antikörper

Produktnummer ABIN7273083

Der Ziege Polyklonal anti-mApple Fluorescent Protein Antikörper wird verwendet zum Nachweis von mApple Fluorescent Protein in Proben von Discosoma. Er wurde validiert für IEM, IF, IHC (fro), IHC (p) und WB.

Kurzübersicht für mApple Fluorescent Protein Antikörper (ABIN7273083)

Target: mApple Fluorescent Protein

Reaktivität: Discosoma

Wirt: Ziege

Klonalität: Polyklonal

Applikation: Immunoelectron Microscopy (IEM), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Produktdetails

Verwendungszweck: anti-mApple

Spezifität: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize GFP (green fluorescent protein).

Kreuzreaktivität (Details): Reacts against Red Fluorescent Protein

Produktmerkmale: Red Fluorescent Protein

Aufreinigung: Epitope affinity purified

Immunogen: Purified recombinant fluorescent protein produced in E. coli.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 3 mg/mL

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: The antibody solution should be gently mixed before use.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

Antigendetails

Target: mApple Fluorescent Protein

Hintergrund:

Goat polyclonal antibody to mApple (Apple fluorescent protein). mApple is a basic (constitutively fluorescent), monomeric engineered derivate of red fluorescent protein (RFP) isolated from members of the Discosoma (mushroom coral) family. mApple is a ~27 kDa protein that is optimally excited at a 568 nm and has a maximum of emission at 592 nm. It is used in research as a reporter to label and study the biology of the cell using a wide range of applications.

red fluorescent protein, Apple antibody.

Molekulargewicht: 27 kDa