BFP Fluorescent Protein Antikörper

Produktnummer ABIN7273077

Der Ziege Polyklonal anti-BFP Fluorescent Protein Antikörper wird verwendet zum Nachweis von BFP Fluorescent Protein in Proben von Aequorea victoria. Er wurde validiert für IEM, IF, IHC (fro), IHC (p) und WB.

Kurzübersicht für BFP Fluorescent Protein Antikörper (ABIN7273077)

Target: BFP Fluorescent Protein

Reaktivität: Aequorea victoria

Wirt: Ziege

Klonalität: Polyklonal

Applikation: Immunoelectron Microscopy (IEM), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Produktdetails

Verwendungszweck: anti-BFP

Spezifität: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize RFP (red fluorescent protein).

Kreuzreaktivität (Details): Reacts against Green Fluorescent Protein

Produktmerkmale: Green Fluorescent Protein

Aufreinigung: Epitope affinity purified

Immunogen: Purified recombinant fluorescent protein produced in E. coli.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 3 mg/mL

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: The antibody solution should be gently mixed before use.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

Antigendetails

Target: BFP Fluorescent Protein

Hintergrund:

Goat polyclonal antibody to BFP (Blue fluorescent protein). BFP is a basic (constitutively fluorescent), monomeric engineered derivate of green fluorescent protein (GFP) isolated from Aequorea victoria. BFP is a ~27 kDa protein that is optimally excited at a 381 nm and has a maximum of emission at 445 nm. It is used in research as a reporter to label and study the biology of the cell using a wide range of applications.

green fluorescent protein antibody.

Molekulargewicht: 27 kDa