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BFP Fluorescent Protein Antikörper

Der Ziege Polyklonal Anti--Antikörper wurde für IEM, IF, IHC (fro), IHC (p) und WB validiert. Er ist geeignet, in Proben von Aequorea victoria zu detektieren.
Produktnummer ABIN7273077

Kurzübersicht für BFP Fluorescent Protein Antikörper (ABIN7273077)

Target

BFP Fluorescent Protein

Reaktivität

Aequorea victoria

Wirt

Ziege

Klonalität

Polyklonal

Applikation

Immunoelectron Microscopy (IEM), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
  • Kreuzreaktivität (Details)

    Reacts against Green Fluorescent Protein

    Aufreinigung

    Epitope affinity purified

    Immunogen

    Purified recombinant fluorescent protein produced in E. coli.

    Isotyp

    IgG
  • Applikationshinweise

    WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500

    Kommentare

    In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize RFP (red fluorescent protein).

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    3 mg/mL

    Buffer

    PBS, 20 % glycerol and 0.05 % sodium azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20 °C for long-term storage. Store at 2-8 °C for up to one month.
  • Target

    BFP Fluorescent Protein

    Hintergrund

    BFP (Blue fluorescent protein) is a basic (constitutively fluorescent), monomeric engineered derivate of green fluorescent protein (GFP) isolated from Aequorea victoria. BFP is a ~27 kDa protein that is optimally excited at a 381 nm and has a maximum of emission at 445 nm. It is used in research as a reporter to label and study the biology of the cell using a wide range of applications.
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