Rekombinanter ALFA-Tag Antikörper (Alexa Fluor 647)

Produktnummer ABIN7272960

Der Alpaca Monoklonal Anti--Antikörper wurde für WB, FACS, ICC, IF und IHC validiert. Er ist geeignet, in Proben von zu detektieren.

Kurzübersicht für Rekombinanter ALFA-Tag Antikörper (Alexa Fluor 647) (ABIN7272960)

Target: ALFA-Tag

Antikörpertyp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Wirt: Alpaca

Klonalität: Monoklonal

Konjugat: Alexa Fluor 647

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)

Klon: 1G5

Produktdetails

Expressionssystem: HEK-293 Cells

Sequenz: SRLEEELRRRLTE

Spezifität: ALFA-tag

Aufreinigung: Produced in: HEK

Immunogen: Single-domain antibody (sdAb) that recognizes a small rationally-designed epitope tag: the ALFA tag.

Labeling Ratio: Two site-specifically conjugated fluorophores per sdAb.

Anwendungsinformationen

Applikationshinweise: 1:500 (corresponding to 5 nM final concentration). ALFA tag and the companion FluoTags-X2 anti-ALFA and ALFA-Catcher are the perfect choices for fluorescent-based experiments that want to be combined with biochemical purifications, mass-spectrometry, or any other high-end application.

Kommentare:

Two site-specifically coupled fluorophores per molecule. The reagent can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of the sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this sdAb can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - renders this product superior tools for all microscopy techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.

Buffer: lyophilized from PBS pH7.4 with 2% BSA (US-Origin)

Lagerung: 4 °C/-20 °C/-80 °C

Informationen zur Lagerung: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.

Antigendetails

Target: ALFA-Tag

Substanzklasse: Tag

Hintergrund: Owing to the small size of our FluoTags and the short ALFA-tag (15 amino acids; PSRLEEELRRRLTEP), the distance between the target epitope and each fluorophore is below 3 nm. Proteins of interest can be tagged with our ALFA-tag at the N-termini, C-termini, or in-between domains and they can also be immunoprecipitated or natively purified with complexes or partner binders using our series of ALFA-Catcher resins with physiological elution capability.