Rekombinanter mRuby Antikörper (Biotin)
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- Target
- mRuby
- Antikörpertyp
- Recombinant Antibody
- Fragment
- single-domain Antibody (sdAb)
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Reaktivität
- Entacmaea quadricolor
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Wirt
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Alpaca
- Expressionssystem
- E.coli
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Klonalität
- Monoklonal
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Konjugat
- Biotin
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Applikation
- Immunofluorescence (IF), Immunocytochemistry (ICC)
- Spezifität
- Recognizes most common mRuby derivatives
- Aufreinigung
- Produced in: E.coli
- Immunogen
- sdAb anti-mRUBY is derived from an in-house developed single-domain antibody (sdAb) that recognizes mRuby and mRuby derivatives, which is a bright monomeric red fluorescent protein derived from Entacmaea quadricolor.
- Klon
- 1F8
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anti-mRuby antibody
Reaktivität: Discosoma IF, IEM, IHC (fro), IHC (p), WB Wirt: Ziege Polyclonal unconjugated
anti-mRuby antibody (Atto 488)Reaktivität: Entacmaea quadricolor IF, ICC, FACS Wirt: Alpaca Monoclonal 1F8 Atto 488 single-domain Antibody (sdAb) Recombinant Antibody
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- Applikationshinweise
- Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Reconsitute with 250 µL 50% glycerol for storage at -80 °C
- Buffer
- sdAbs are lyophilized from PBS pH 7.4.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.
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- Target
- mRuby
- Hintergrund
- MRuby is a bright monomeric red fluorescent protein, which has more than 20 mutations compared to its wild-type predecessor (eqFP611) found in Entacmaea quadricolor . mRuby, mRuby2 & mRuby3 are all exited at 560 nm, with mRuby3 being the brightest.
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