Rekombinanter mEos Antikörper (Biotin)

Produktnummer ABIN7272888

Der Alpaca Monoklonal anti-mEos Antikörper wird verwendet zum Nachweis von mEos in Proben von Lobophyllia hemprichii. Er wurde validiert für ICC und IF.

Kurzübersicht für Rekombinanter mEos Antikörper (Biotin) (ABIN7272888)

Target: mEos

Antikörpertyp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reaktivität: Lobophyllia hemprichii

Wirt: Alpaca

Klonalität: Monoklonal

Konjugat: Biotin

Applikation: Immunocytochemistry (ICC), Immunofluorescence (IF)

Klon: 1E8

Produktdetails

Expressionssystem: E.coli

Spezifität: Recognizes mEOS and mEOS derivatives

Aufreinigung: Produced in: E.coli

Immunogen: sdAb anti-mEOS is derived from an in-house developed single-domain antibody (sdAb) that recognizes mEOS, which is a bright monomeric green photoswitchable fluorescent protein derived from Lobophyllia hemprichii.

Anwendungsinformationen

Applikationshinweise: Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconsitute with 250 µL 50% glycerol for storage at -80 °C

Buffer: sdAbs are lyophilized from PBS pH 7.4.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.

Antigendetails

Target: mEos

Hintergrund: In 2004 Wiedernmann and colleagues described an engineered fluorescent protein with photoswitchable properties, EosFP, which could be selectively shifted from green to red emission and was derived from a fluorescent protein discovered in Lobopyllia hemprichii. The same study also describes a monomeric, improved version, mEOS. Over the last years, several improved versions were discovered expanding the repertoir of photoswitchable fluorescent probes.