Rekombinanter SYT1 Antikörper (Atto 488)

Produktnummer ABIN7272874

Dieser Anti-SYT1 Antikörper ist ein Alpaca Monoklonal Antikörper zur Detektion von SYT1 in IF und ICC. Geeignet für Maus.

Kurzübersicht für Rekombinanter SYT1 Antikörper (Atto 488) (ABIN7272874)

Target: SYT1 (Synaptotagmin I (SYT1))

Antikörpertyp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reaktivität: Maus

Wirt: Alpaca

Klonalität: Monoklonal

Konjugat: Dieser SYT1 Antikörper ist konjugiert mit Atto 488

Applikation: Immunofluorescence (IF), Immunocytochemistry (ICC)

Klon: 7D5

Produktdetails anti-SYT1 Antikörper (Atto 488)

Expressionssystem: E.coli

Spezifität: Synaptotagmin-1

Aufreinigung: Produced in: E.coli

Immunogen: anti-Synaptotagmin 1 (SYT1) is an alpaca single-domain antibody (sdAb) that recognizes specifically the cytoplasmic domain of murine Synaptotagmin 1 with high affinity and specificity.

Labeling Ratio: Two site-specifically conjugated fluorophores per sdAb.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Two site-specifically coupled fluorophores per molecule. The reagent can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of the sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this sdAb can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - renders this product superior tools for all microscopy techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.

Buffer: lyophilized from PBS pH7.4 with 2% BSA (US-Origin)

Lagerung: 4 °C/-20 °C/-80 °C

Informationen zur Lagerung: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.

Details zu SYT1

Target: SYT1 (Synaptotagmin I (SYT1))

Andere Bezeichnung: Synaptotagmin-1

Hintergrund: Synaptotagmin 1 (Syt1) belongs to a family with 17 members. Synaptotagmins are all single-pass transmembrane proteins containing two domains binding to calcium at their intracellular portion. Synaptotagmin 1 is enriched at the pre-synaptic boutons on axon terminals, associated with synaptic vesicles, and is implicated in triggering vesicle fusion and neurotransmitter release upon a local increase of calcium concentration.

Pathways: Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport