Rekombinanter DLG4 Antikörper (Atto 488)

Produktnummer ABIN7272871

Dieser Anti-DLG4 Antikörper ist ein Alpaca Monoklonal Antikörper zur Detektion von DLG4 in WB, IHC, ICC und IF. Geeignet für Ratte und Maus.

Kurzübersicht für Rekombinanter DLG4 Antikörper (Atto 488) (ABIN7272871)

Target: DLG4 (Discs, Large Homolog 4 (Drosophila) (DLG4))

Antikörpertyp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reaktivität: Ratte, Maus

Wirt: Alpaca

Klonalität: Monoklonal

Konjugat: Dieser DLG4 Antikörper ist konjugiert mit Atto 488

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Klon: 1B2

Produktdetails anti-DLG4 Antikörper (Atto 488)

Expressionssystem: E.coli

Spezifität: Recognizes mouse and rat PSD95. Other species not tested

Aufreinigung: Produced in: E.coli

Immunogen: anti-PSD95 is a fluorescently conjugated single-domain antibody that recognizes mouse and rat PSD95.

Labeling Ratio: Two site-specifically conjugated fluorophores per sdAb.

Anwendungsinformationen

Applikationshinweise: IF: 1:500 (corresponding to 5 nM final concentration) , 5 µg/mL (IHC),

Kommentare:

Two site-specifically coupled fluorophores per molecule. The reagent can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of the sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this sdAb can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - renders this product superior tools for all microscopy techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.

Buffer: lyophilized from PBS pH7.4 with 2% BSA (US-Origin)

Lagerung: 4 °C/-20 °C/-80 °C

Informationen zur Lagerung: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.

Details zu DLG4

Target: DLG4 (Discs, Large Homolog 4 (Drosophila) (DLG4))

Andere Bezeichnung: PSD95

Hintergrund: PSD95 is a member of the MAGUK family, and it plays an important role in the formation of a dynamic scaffold at the post synapses. It functions as an organizing centrum of receptors, ion channels, kinases, and other relevant players in achieving synaptic plasticity and molecular changes during long-term potentiation.

Pathways: Regulation of Muscle Cell Differentiation, Synaptic Membrane, Skeletal Muscle Fiber Development, Asymmetric Protein Localization, Regulation of long-term Neuronal Synaptic Plasticity