Rekombinanter SARS-CoV-2 Nucleocapsid Antikörper (Alexa Fluor 568)

Produktnummer ABIN7272860

Dieser Alpaca Monoklonal Antikörper erkennt spezifisch SARS-CoV-2 Nucleocapsid in IF und ICC. Er zeigt eine Reaktivität gegenüber SARS Coronavirus-2 (SARS-CoV-2).

Kurzübersicht für Rekombinanter SARS-CoV-2 Nucleocapsid Antikörper (Alexa Fluor 568) (ABIN7272860)

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Antikörpertyp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reaktivität: SARS Coronavirus-2 (SARS-CoV-2)

Wirt: Alpaca

Klonalität: Monoklonal

Konjugat: Dieser SARS-CoV-2 Nucleocapsid Antikörper ist konjugiert mit Alexa Fluor 568

Applikation: Immunofluorescence (IF), Immunocytochemistry (ICC)

Klon: 2A7

Produktdetails anti-SARS-CoV-2 Nucleocapsid Antikörper (Alexa Fluor 568)

Expressionssystem: E.coli

Spezifität: Nucleocapsid from SARS-CoV-2 and SARS-CoV-1

Aufreinigung: Produced in: E.coli

Labeling Ratio: a single fluorophore is coupled to exactly one sdAb

Anwendungsinformationen

Applikationshinweise: IF: 1:500 (Our nanobody binds strongly to protein N of SARS-CoV-2, even under harsh sample treatment conditions, e.g., paraffin-embedded tissue.)

Kommentare:

Each fluorophore is coupled to exactly one sdAb, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.

Buffer: lyophilized from PBS pH7.4 with 2% BSA (US-Origin)

Lagerung: 4 °C/-20 °C/-80 °C

Informationen zur Lagerung: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.

Details zu SARS-CoV-2 Nucleocapsid

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Andere Bezeichnung: Nucleocapsid from SARS-CoV-2

Hintergrund: The nucleocapsid (N) protein of SARS-CoV-2 is an abundant RNA-binding protein critical for the packaging of the single-stranded RNA viral genome. Due to its abundance, it is an interesting target for antibody detection in biopsies and tissue. The protein N is also present inside virion particles bound to single-stranded RNA.