tau Antikörper (AA 297-391) (APC)

Produktnummer ABIN7272119

Der Kaninchen Polyklonal anti-tau Antikörper wird verwendet zum Nachweis von tau in Proben von Human. Er wurde validiert für WB.

Kurzübersicht für tau Antikörper (AA 297-391) (APC) (ABIN7272119)

Target: tau (tau Protein)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser tau Antikörper ist konjugiert mit APC

Applikation: Western Blotting (WB)

Produktdetails anti-tau Antikörper (APC)

Bindungsspezifität: AA 297-391

Verwendungszweck: Rabbit Anti-Human Truncated Tau Fragment (AA297-391) (dGAE) Polyclonal,

Spezifität: Widely expressed, with high levels in brain.,Detects ~10.2 kDa.

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Protein A

Immunogen: Recombinant Human Tau AA297-391 (dGAE) Fibril (SPR-461)

Anwendungsinformationen

Applikationshinweise: WB (1:1000), optimal dilutions for assays should be determined by the user.

Kommentare:

A 1:1000 dilution was sufficient for detection of tau in 15 μg of mouse, rat brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu tau

Target: tau (tau Protein)

Hintergrund: Alzheimer's Disease (AD) is the most common neurodegenerative disease, affecting 10 % of seniors over the age of 65 (1). It was named after Alois Alzheimer, a German scientist who discovered tangled bundles of fibrils where neurons had once been in the brain of a deceased patient in 1907 (2). Tau (tubulin-associated unit) is normally located in the axons of neurons where it stabilizes microtubules. Tauopathies such as AD are characterized by neurofibrillary tangles containing paired helical filaments (PHFs). A truncated 95-amino acid fragment corresponding to residues 297-391 of full-length tau has been shown to assemble into PHF-like fibrils in vitro in the absence of additives or templates (3). This fragment has been found in the core of PHFs from AD brains and forms filaments that closely resemble PHFs isolated from AD brains (3)., cellular localisation: Cytoplasm, Axolemma, Axolemma Plasma Membrane, Axon, Cell Body, Cell membrane, Cytoplasmic Ribonucleoprotein Granule, Cytoplasmic Side, Cytoskeleton, Cytosol, Dendrite, Growth cone, Microtubule, Microtubule Associated Complex, Neurofibrillary Tangle, Neuronal Cell Body, Nuclear Periphery, Nuclear Speck, Nucleus, Peripheral membrane protein, Plasma Membrane, Tubulin Complex

Gen-ID: 4137

NCBI Accession: NP_005901

UniProt: P10636