Rekombinanter SARS-CoV-2 Spike Antikörper (RBD) (Biotin)

Produktnummer ABIN7273331

Dieses Anti-SARS-CoV-2 Spike-Antikörper ist ein Maus Chimeric-Antikörper zur Detektion von SARS-CoV-2 Spike in ELISA, Neut und SR. Geeignet für SARS Coronavirus-2 (SARS-CoV-2).

Kurzübersicht für Rekombinanter SARS-CoV-2 Spike Antikörper (RBD) (Biotin) (ABIN7273331)

Target: SARS-CoV-2 Spike

Antikörpertyp: Recombinant Antibody

Reaktivität: SARS Coronavirus-2 (SARS-CoV-2)

Wirt: Maus

Klonalität: Chimeric

Konjugat: Dieser SARS-CoV-2 Spike Antikörper ist konjugiert mit Biotin

Applikation: ELISA, Neutralization (Neut), Spike and Recovery (SR)

Güteklasse: MALS verified

Klon: AM122

Produktdetails anti-SARS-CoV-2 Spike Antikörper (Biotin)

Bindungsspezifität: RBD

Verwendungszweck: Biotinylated Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM122) (MALS verified)

Spezifität: This product is a specific antibody against SARS-CoV-2 Spike protein RBD domain. No cross-reactivity is detected with Spike RBD domain of other coronaviruses, including SARS-CoV, MERS-CoV, HCoV-229E, HCoV-NL63, HCoV-OC43 and HCoV-HKU1.

Produktmerkmale: Recombinant Antibodies produced in HEK293. Biotinylated Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Human IgG1. Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (S1N-M122) is a chimeric monoclonal antibody recombinantly expressed from HEK293 cells, which combines the variable region of a mouse monoclonal antibody with human IgG1 constant domain. The mouse monoclonal antibody was obtained from a mouse immunized with recombinant SARS-CoV-2 Spike S1 Protein. As verified in competitive ELISA-based and pseudovirus-based neutralization assay, this chimeric monoclonal can potently neutralize all SARS-CoV-2 Variants of Concern (VOCs), including Alpha (B.1.1.7), Beta (B.1.351), Gamma (P.1) and Delta (B.1.617.2).

Immunogen: Biotinylated Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Human IgG1 (ABIN7271742 and ABIN7273331) Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (ABIN6953206 and ABIN6953211) is a chimeric monoclonal antibody recombinantly expressed from HEK293 cells, which combines the variable region of a mouse monoclonal antibody with human IgG1 constant domain. The mouse monoclonal antibody was obtained from a mouse immunized with recombinant SARS-CoV-2 Spike S1 Protein. As verified in competitive ELISA-based and pseudovirus-based neutralization assay, this chimeric monoclonal can potently neutralize all SARS-CoV-2 Variants of Concern (VOCs), including Alpha (B.1.1.7), Beta (B.1.351), Gamma (P.1) and Delta (B.1.617.2).

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: This antibody can be paired with other Anti-SARS-CoV-2 Spike S1 antibodies to detect SARS-CoV-2 Spike S1 protein in sandwich ELISA or LFA assay.

Kommentare:

Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Powder

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Details zu SARS-CoV-2 Spike

Target: SARS-CoV-2 Spike

Hintergrund: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.