NHE7 Antikörper (Extracellular, Loop 2)

Produktnummer ABIN7886819

Der Kaninchen Polyklonal anti-NHE7 Antikörper (ABIN7886819) detektiert spezifisch NHE7 in WB und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Maus.

Kurzübersicht für NHE7 Antikörper (Extracellular, Loop 2) (ABIN7886819)

Target: NHE7 (SLC9A7) (Sodium Hydrogen Exchanger 7 (SLC9A7))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NHE7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-NHE7 Antikörper

Bindungsspezifität: AA 158-171, Extracellular, Loop 2

Verwendungszweck: A Rabbit Polyclonal antibody to Na+/H+ Exchanger 7 (NHE7) (extracellular)

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Rat - identical, Human - 13 out of 14 amino acid residues identical

Produktmerkmale: Extracellular, 2nd loop

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)EISPGKINNVEQND, corresponding to amino acid residues 158 - 171 of mouse SLC9A7 

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: 5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7236882)

Blocking Peptide: (ABIN7236882)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu NHE7

Target: NHE7 (SLC9A7) (Sodium Hydrogen Exchanger 7 (SLC9A7))

Andere Bezeichnung: SLC9A7

Hintergrund:

Synonyms: Sodium/hydrogen exchanger 7, Solute Carrier Family 9 Member A7, SLC9A7, NHE-7

Description: Na+/H+ exchangers (NHEs) also termed solute carrier family 9 (SLC9), are proteins that function as ion transporters. NHEs are present across all kingdoms of life from prokaryotes to eukaryotes and are found to be crucial in preventing intracellular acidification. NHEs are divided into 3 groups SLC9A (NHE1-9), SLC9B (NHA1 and NHA2) and SLC9C according to their cellular location and their function. NHEs regulates cell volume and pH of the cytosol and organelles1.NHE7 is an NHE that is localized predominantly to the trans-Golgi network (TGN) and post-Golgi vesicles. NHE7 differ from other NHEs by its ability to transport both Na+ and K+ via a H+ gradient-dependent manner2. NHE7 mRNA is expressed in wide range of cells but is especially abundant in the brain, skeletal muscle and various secretory tissues3. The predicted membrane topology of NHE7 consist of 12 transmembrane domains (TMDs) and a single N-glycosylation site at position 145. Similarly to the other SLC9A isoforms NHE7 can be assembled as a homodimer. It was recently found that a missense variant in NHE7 associates with multigenerational nonsyndromic intellectual disability (ID)3.

Gen-ID: 84679

NCBI Accession: NM_032591

UniProt: Q96T83