NHE7 Antikörper (Extracellular)

Produktnummer ABIN7237818

Dieses Anti-NHE7-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von NHE7 in WB und IHC. Geeignet für Maus.

Kurzübersicht für NHE7 Antikörper (Extracellular) (ABIN7237818)

Target: NHE7 (SLC9A7) (Sodium Hydrogen Exchanger 7 (SLC9A7))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NHE7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-NHE7 Antikörper

Bindungsspezifität: AA 158-171, Extracellular

Verwendungszweck: A Rabbit Polyclonal antibody to Na+/H+ Exchanger 7 (NHE7) (extracellular)

Spezifität: Extracellular, 2nd loop.

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Rat - identical, Human - 13 out of 14 amino acid residues identical

Produktmerkmale: Extracellular, 2nd loop

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EISPGKINNVEQND, corresponding to amino acid residues 158 - 171 of mouse SLC9A7

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: (ABIN7236882)

Blocking Peptide: (ABIN7236882)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu NHE7

Target: NHE7 (SLC9A7) (Sodium Hydrogen Exchanger 7 (SLC9A7))

Andere Bezeichnung: SLC9A7

Hintergrund: Sodium/hydrogen exchanger 7, Solute Carrier Family 9 Member A7, SLC9A7, NHE-7,Na+/H+ exchangers (NHEs) also termed solute carrier family 9 (SLC9), are proteins that function as ion transporters. NHEs are present across all kingdoms of life from prokaryotes to eukaryotes and are found to be crucial in preventing intracellular acidification. NHEs are divided into 3 groups SLC9A (NHE1-9), SLC9B (NHA1 and NHA2) and SLC9C according to their cellular location and their function. NHEs regulates cell volume and pH of the cytosol and organelles1.NHE7 is an NHE that is localized predominantly to the trans-Golgi network (TGN) and post-Golgi vesicles. NHE7 differ from other NHEs by its ability to transport both Na+ and K+ via a H+ gradient-dependent manner2. NHE7 mRNA is expressed in wide range of cells but is especially abundant in the brain, skeletal muscle and various secretory tissues3. The predicted membrane topology of NHE7 consist of 12 transmembrane domains (TMDs) and a single N-glycosylation site at position 145. Similarly to the other SLC9A isoforms NHE7 can be assembled as a homodimer. It was recently found that a missense variant in NHE7 associates with multigenerational nonsyndromic intellectual disability (ID)3.

Alternative names: Sodium/hydrogen exchanger 7, Solute Carrier Family 9 Member A7, SLC9A7, NHE-7

Gen-ID: 84679

NCBI Accession: NM_032591

UniProt: Q96T83