NEO1 Antikörper (Extracellular, N-Term)

Produktnummer ABIN7886702

Der Kaninchen Polyklonal anti-NEO1 Antikörper (ABIN7886702) detektiert spezifisch NEO1 in WB, IHC, FACS und LCI. Dieser Antikörper reagiert spezifisch mit Proben aus Maus.

Kurzübersicht für NEO1 Antikörper (Extracellular, N-Term) (ABIN7886702)

Target: NEO1 (Neogenin 1 (NEO1))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NEO1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Produktdetails anti-NEO1 Antikörper

Bindungsspezifität: AA 701-715, Extracellular, N-Term

Verwendungszweck: A Rabbit Polyclonal antibody to Neogenin (extracellular)

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Human,rat - identical

Produktmerkmale: Extracellular, N-terminus

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)KIRYRKASRKSDVTE, corresponding to amino acid residues 701 - 715 of mouse Neogenin

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235994)

Blocking Peptide: (ABIN7235994)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu NEO1

Target: NEO1 (Neogenin 1 (NEO1))

Andere Bezeichnung: NEO1

Hintergrund:

Synonyms: Neogenin 1, NEO1, NGN

Description: Neogenin also known as NEO1 is a member of the immunoglobulin (Ig) superfamily. It has been reported to be involved in diverse physiology and pathology functions, including cell proliferation, differentiation and migration1. Neogenin was originally isolated from embryonic chicken cerebellum and reported to be homologous to the axon guidance receptor deleted in colorectal cancer (DCC)2.Neogenin consists of 4 N-terminal Ig-like domains, followed by 6 fibronectin type III-like domains, a single transmembrane helix, and an intracellular domain. Neogenin is capable to mediate attractive and repulsive axon guidance depending on the ligand identity. Two main ligands of neogenin are Netrin-1 (NET1) and repulsive guidance molecules (RGMs)3.Interaction of Neogenin with RGMs leads to cytoskeleton rearrangements via Rho GTPases, this results in growth cone collapse. In contrast interaction with Netrin-1 triggers attractive growth cone response3. Recently it was shown that Neogenin plays a role in the maintenance of iron homeostasis via interaction with HJV (members of the RGM family)4.

Gen-ID: 18007

NCBI Accession: NM_002499

UniProt: P97798

Pathways: Transition Metal Ion Homeostasis, Regulation of Muscle Cell Differentiation, Tube Formation