HCAR2 Antikörper (Extracellular, Loop 1)

Produktnummer ABIN7886508

Der Kaninchen Polyklonal anti-HCAR2 Antikörper (ABIN7886508) detektiert spezifisch HCAR2 in WB, IHC, FACS und LCI. Dieser Antikörper reagiert spezifisch mit Proben aus Maus.

Kurzübersicht für HCAR2 Antikörper (Extracellular, Loop 1) (ABIN7886508)

Target: HCAR2 (Hydroxycarboxylic Acid Receptor 2 (HCAR2))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HCAR2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Produktdetails anti-HCAR2 Antikörper

Bindungsspezifität: AA 82-94, Extracellular, Loop 1

Verwendungszweck: A Rabbit Polyclonal antibody to GPR109A/HCAR2 (extracellular)

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Rat - 11 out of 13 amino acid residues identical, Human - 9 out of 13 amino acid residues identical Note: it is not expected to recognize the highly homologous human protein HCAR3 (Accession P49019) HCAR3 has no mouse,rat orthologs

Produktmerkmale: Extracellular, 1st loop.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)DNYVHNWDWRFGG, corresponding to amino acid residues 82 - 94 of mouse HCAR2 

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235778)

Blocking Peptide: (ABIN7235778)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu HCAR2

Target: HCAR2 (Hydroxycarboxylic Acid Receptor 2 (HCAR2))

Andere Bezeichnung: HCAR2

Hintergrund:

Synonyms: Hydroxycarboxylic Acid Receptor 2, Niacin Receptor 1, G-Protein Coupled Receptor HM74A, HM74A, Nicotinic acid receptor, PUMAG

Description: Hydroxy-carboxylic acid receptors (HCA1-3) are a family of G-protein-coupled receptors that regulate lipolysis. HCAR2 (GPR109A) was identified as the receptor for nicotinic acid (niacin, vitamin B3) and beta-hyroxybutyrate (β-OHB), a ketone body synthesized by the liver under negative energy balance1.HCAR2 is mainly expressed on adipocytes and immune cells and in lower levels in retinal pigmented and colonic epithelial cells, microglia and normal mammary tissue. High levels of HCAR2 can be found in immune cells such as macrophages in response to the presence of interferon-γ, TNF-α and hypoxia2.HCAR2 activity in adipocytes promotes inhibition of adenylate cyclase and decrease cAMP levels. HCAR2 activity leads to a rapid decrease in lipolysis and reduction in the release of fatty acids from the adipocytes3.Activation of HCAR2 has also been associated with anti-inflammatory effects in different disease states such as sepsis, diabetes and obesity. Interaction of HCAR2 with niacin and β-OHB under this conditions lead to variety of signaling events involving inflammatory downstream targets4.

Gen-ID: 80885

NCBI Accession: NM_177551

UniProt: Q9EP66