GPR97 Antikörper (Extracellular, N-Term)

Produktnummer ABIN7886447

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch GPR97 in WB, IHC, FACS und LCI. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für GPR97 Antikörper (Extracellular, N-Term) (ABIN7886447)

Target: GPR97 (G Protein-Coupled Receptor 97 (GPR97))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPR97 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Produktdetails anti-GPR97 Antikörper

Bindungsspezifität: AA 62-75, Extracellular, N-Term

Verwendungszweck: A Rabbit Polyclonal antibody to GPR97 (ADGRG3) (extracellular)

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse,rat - 12 out of 14 amino acid residues identical

Produktmerkmale: Extracellular, N-term

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: CNVENLQRYWLNYE, corresponding to amino acid residues 62 - 75 of human ADGRG3

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235457)

Blocking Peptide: (ABIN7235457)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu GPR97

Target: GPR97 (G Protein-Coupled Receptor 97 (GPR97))

Andere Bezeichnung: ADGRG3 

Hintergrund:

Synonyms: Adhesion G Protein-Coupled Receptor G3, G-protein coupled receptor 97

Description: Adhesion G protein-coupled receptors (aGPCRs) are one of the five main families in the GPCR superfamily. They act as a molecular switch that regulate many physiological processes, including brain development, ion-water homeostasis, inflammation and cell-fate determination1.One member of the aGPCRs family is the orphan receptor GPR97 (also known as ADGRG3). GPR97 consist of seven-transmembrane domain and a GPCR-Autoproteolysis INducing (GAIN) domain. Auto-cleavage via the GAIN domain produces two subunits: the α-subunit (N-terminal fragment (NTF)) and the β-subunit (C-terminal fragment) that remain non-covalently associated at the cell surface2. GPR97 is mainly expressed in immune cells such as neutrophils, eosinophils, and mast cells. Activation of GPR97 results in reduction in cAMP levels in combination with an increase in downstream effectors of Gβγ, such as SRE and NF-κB3. GPR97 was found to regulate B-cell development in mice and antimicrobial activity in human granulocytes3. In addition, studies have shown that GPR97 is involved in macrophage inflammation in high fat diet-induced obesity in mice4.

Gen-ID: 222487

NCBI Accession: NM_170776

UniProt: Q86Y34