GPR142 Antikörper (Extracellular, Loop 3)

Produktnummer ABIN7886464

Der Kaninchen Polyklonal anti-GPR142 Antikörper wird verwendet zum Nachweis von GPR142 in Proben von Maus. Er wurde validiert für WB, FACS, LCI und IHC.

Kurzübersicht für GPR142 Antikörper (Extracellular, Loop 3) (ABIN7886464)

Target: GPR142 (G Protein-Coupled Receptor 142 (GPR142))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPR142 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI), Immunohistochemistry (IHC)

Produktdetails anti-GPR142 Antikörper

Bindungsspezifität: AA 291-304, Extracellular, Loop 3

Verwendungszweck: A Rabbit Polyclonal antibody to GPR142 (extracellular)

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Rat,human - identical

Produktmerkmale: Extracellular, 3rd loop

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)APVHRDWRVHLALD, corresponding to amino acid residues 291 - 304 of mouse GPR142

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:400

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:1000

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235372)

Blocking Peptide: (ABIN7235372)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu GPR142

Target: GPR142 (G Protein-Coupled Receptor 142 (GPR142))

Andere Bezeichnung: GPR142

Hintergrund:

Synonyms: G-Protein Coupled Receptor 142, Probable G-Protein Coupled Receptor 142, PGR2

Description: G-protein-coupled receptors (GPCRs) are the largest family of membrane proteins and mediate a myriad of physiological and pathophysiological signaling processes. Of these receptors, all are seven membrane spanning receptors but not all are G protein binding but it is convenient to refer to the receptors as GPCRs1.The GPR142 is a GPCR selectively activated by aromatic amino acids and share 33 % amino acid identity with GPR139. L-tryptophan representing one of the most potent ligands, having an EC50 values of 0.2 - 1 mM2. GPR142 is expressed in both rodent and human pancreatic beta cells. Moreover, it was found to be implicated in the regulation of insulin, thereby having a crucial role in Type II diabetes management3. Moreover, GPR142 is highly expressed in immune cells and its ligand L-tryptophan and metabolite serotonin are known to take part in inflammation process. It was shown that mice lacking GPR142 gene are more resilient to anti-type II collagen (CII) antibody-induced arthritis however the exact mechanism is currently unknown4.

Gen-ID: 217302

NCBI Accession: NM_181790

UniProt: Q7TQN9