GPR139 Antikörper (Intracellular, Loop 3)

Produktnummer ABIN7886456

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch GPR139 in IHC und WB. Es zeigt Reaktivität gegenüber Proben von Maus.

Kurzübersicht für GPR139 Antikörper (Intracellular, Loop 3) (ABIN7886456)

Target: GPR139 (G Protein-Coupled Receptor 139 (GPR139))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPR139 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB)

Produktdetails anti-GPR139 Antikörper

Bindungsspezifität: AA 204-217, Intracellular, Loop 3

Verwendungszweck: A Rabbit Polyclonal antibody to Parkin

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Rat,Human - identical

Produktmerkmale: Intracellular, 3rd loop

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)RKSNFRLRGYSTGK, corresponding to amino acid residues 204 - 217 of mouse GPR139 

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200-1:800

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:600

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235369)

Blocking Peptide: (ABIN7235369)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu GPR139

Target: GPR139 (G Protein-Coupled Receptor 139 (GPR139))

Andere Bezeichnung: GPR139 

Hintergrund:

Synonyms: G Protein-Coupled Receptor 139, PGR3, G(Q)-Coupled Orphan Receptor GPRg1, GPRG1

Description: G-protein-coupled receptors (GPCRs), are the largest family of membrane proteins and mediate most of the physiological responses to hormones, neurotransmitters and environmental stimulants. At the most basic level, all GPCRs are characterized by the presence of seven membrane-spanning α-helical segments separated by alternating intracellular and extracellular loop regions1. Orphan GPCRs are a group of GPCRs that their endogenous ligands remain unidentified and therefore their natural functions in doubt2.GPR139 is an orphan GPCRs for which amino acids L-tryptophan and L-phenylalanine, the adrenocorticotropic hormone (ACTH), and α- and β-melanocyte-stimulating hormone have been proposed as endogenous agonists3. GPR139 was found to be highly conserved across species, as the amino acid sequence of the human GPR139 protein is 96 % , 92 % and 70 % identical to the mouse, chicken and zebrafish orthologs, respectively. According to mRNA expression levels GPR139 is mainly expressed in the central nervous system (CNS). GPR139 has been reported to activate several G protein pathways of which Gq/11 is the primary one4.

Gen-ID: 209776

NCBI Accession: NM_001002911

UniProt: Q80UC8