Digoxigenin Antikörper

Produktnummer ABIN7233195

Der Maus Monoklonal anti-Digoxigenin Antikörper (Klon 21H8) (ABIN7233195) detektiert spezifisch Digoxigenin in ELISA, WB und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Digitalis purpurea (Common foxglove).

Kurzübersicht für Digoxigenin Antikörper (ABIN7233195)

Target: Digoxigenin (DIG)

Reaktivität: Digitalis purpurea (Common foxglove)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Digoxigenin Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Klon: 21H8

Produktdetails anti-Digoxigenin Antikörper

Verwendungszweck: Anti-Digoxigenin Mouse Monoclonal Antibody

Spezifität: This antibody reacts with free and bound digoxigenin.

Kreuzreaktivität: Digitalis purpurea (Common foxglove)

Immunogen: Digoxigenin conjugate.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: use at 0.1-1.0 μg/mL
Immunoblotting:
User should use at 2-5 μg/mL.
Immunohistochemistry: Use at 1-5 μg/mL.
These are recommended concentrations.
End user should determine optimal concentrations for their applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute lyophilized antibody using ultrapure to a final concentration no less than 100 μg/mL. Reconstitution with PBS or Tris buffer is acceptable if required. Protein carrier, biocide or cryopreservative can be added as needed. Aliquot and store at -20C for longterm storage. Dilute immediately prior to use.

Buffer: 0.1M Tris, 0.1M glycine, 2 % sucrose

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.

Details zu Digoxigenin

Target: Digoxigenin (DIG)

Andere Bezeichnung: Digoxigenin

Substanzklasse: Chemical

Hintergrund: Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea, Digitalis orientalis and Digitalis lanata (foxgloves). It is used in many molecular biology applications similarly to other popular haptens such as DNP (dinitrophenol), biotin, and fluorescein and is often used as an immunohistochemical marker for in situ hybridization. In this case it is conjugated to a single species of RNA nucleotide triphosphate (typically uridine), which is then incorporated into RNA as it is synthesized.

CAS-Nummer: 1672-46-4