Rho-related GTP-binding protein Antikörper

Produktnummer ABIN7233029

Der Maus Monoklonal Anti-Rho-related GTP-binding protein-Antikörper wurde für ELISA, WB, IHC, IP und IF/ICC validiert. Er ist geeignet, Rho-related GTP-binding protein in Proben von Säugetier zu detektieren.

Kurzübersicht für Rho-related GTP-binding protein Antikörper (ABIN7233029)

Target: Rho-related GTP-binding protein (RhO (pan))

Reaktivität: Säugetier

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Rho-related GTP-binding protein Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (fixed cells) (IF/ICC)

Klon: 4D2

Produktdetails anti-Rho-related GTP-binding protein Antikörper

Verwendungszweck: Anti-Rhodopsin Mouse Monoclonal Antibody

Spezifität: This antibody recognizes the N-terminus of Rhodopsin from all mammalian species tested (human, mouse, rat, bovine, porcine), and most fish, birds, and amphibians.

Kreuzreaktivität: Amphibien, Avian, Fisch, Säugetier

Immunogen: Bovine rhodopsin

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunoblotting: use at 1-10 μg/mL. A band of ~40 kDa is detected.
Immunohistochemistry: use at 1-10 μg/mL, paraffin-embedded and frozen.
Immunoprecipitation: use at 1-2 μg per 100-500 μg of protein.
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: Rat eye lysate

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Konzentration: Lot specific

Buffer: PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: This antibody is stable for at least one (1) year at -20°C.

Details zu Rho-related GTP-binding protein

Target: Rho-related GTP-binding protein (RhO (pan))

Andere Bezeichnung: RHO

Hintergrund: Rhodopsin,Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth (By similarity). Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change that activates signaling via G-proteins (PubMed:10926528, PubMed:12044163, PubMed:11972040, PubMed:16908857, PubMed:16586416, PubMed:17060607, PubMed:17449675, PubMed:18818650, PubMed:21389983, PubMed:22198838, PubMed:23579341, PubMed:25205354, PubMed:27458239). Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling (PubMed:1396673, PubMed:15111114). {UniProtKB:P08100, PubMed:1396673, PubMed:16586416, PubMed:16908857, PubMed:17060607, PubMed:17449675, PubMed:18818650, PubMed:21389983, PubMed:22198838, PubMed:23579341, PubMed:25205354, PubMed:27458239, PubMed:10926528, PubMed:11972040, PubMed:12044163, PubMed:15111114, PubMed:26526852}.,Rhodopsin is comprised of the protein opsin and a reversibly covalently bound cofactor, retinal. Opsin, made up of seven membrane-embedded alpha helices, binds retinal, a photoreactive chromophore, in a central pocket. In addition to being the pigment of the retina that is responsible for the formation of photoreceptor cells, rhodopsin conveys information stored in the chromophore to the surface of the molecule upon light absorption. Mutations in the rhodopsin gene lead to retinitis pigmentosa.,Membrane, Cell projection, cilium, photoreceptor outer segment

UniProt: P02699