Topo IIalpha (AA 1-80) Antikörper

Produktnummer ABIN7217356

Der Kaninchen Polyklonal anti-Topo IIalpha Antikörper (ABIN7217356) detektiert spezifisch Topo IIalpha in WB, ELISA, IHC (p) und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Affe.

Kurzübersicht für Topo IIalpha (AA 1-80) Antikörper (ABIN7217356)

Target: Topo IIalpha

Reaktivität: Human, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Produktdetails

Bindungsspezifität: AA 1-80

Verwendungszweck: Topo IIα Polyclonal Antibody

Spezifität: Topo IIα Polyclonal Antibody detects endogenous levels of Topo IIα protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the N-terminal region of human Topo IIalpha at AA range: 1-80

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.

Kommentare:

Primary Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Antigendetails

Target: Topo IIalpha

Hintergrund: Rabbit Anti-Topo IIα Polyclonal Antibody,TOP2A, TOP2, DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme,TOP2A encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.,DNA topoisomerase 2-alpha

Molekulargewicht: observerd band 174kDa

Gen-ID: 7153

UniProt: P11388