TJP1 Antikörper

Produktnummer ABIN7232246

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch TJP1 in WB, IF und ELISA. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Schwein.

Kurzübersicht für TJP1 Antikörper (ABIN7232246)

Target: TJP1 (Tight Junction Protein 1 (TJP1))

Reaktivität: Human, Maus, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TJP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), ELISA

Produktdetails anti-TJP1 Antikörper

Verwendungszweck: Rabbit Anti-ZO1 Polyclonal Antibody

Spezifität: The antibody detects endogenous levels of ZO1 protein

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human ZO1 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IF 1:100-300,ELISA 1:5000-20000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu TJP1

Target: TJP1 (Tight Junction Protein 1 (TJP1))

Andere Bezeichnung: ZO1

Hintergrund: Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1TJP1 (Tight Junction Protein 1) encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molekulargewicht: 192kD

Gen-ID: 7082

UniProt: Q07157

Pathways: Carbohydrate Homeostasis, Cell-Cell Junction Organization