Shugoshin Antikörper (pSer14)

Produktnummer ABIN7219856

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Shugoshin in ELISA, IHC und IF. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für Shugoshin Antikörper (pSer14) (ABIN7219856)

Target: Shugoshin (SGOL1) (Shugoshin 1 (SGOL1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Shugoshin Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-Shugoshin Antikörper

Bindungsspezifität: pSer14

Verwendungszweck: Rabbit Anti-Sgo1 (phospho Ser14) Polyclonal Antibody

Spezifität: Phospho-Sgo1 (S14) Polyclonal Antibody detects endogenous levels of Sgo1 protein only when phosphorylated at S14.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Sgo1 Phospho-Ser14

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu Shugoshin

Target: Shugoshin (SGOL1) (Shugoshin 1 (SGOL1))

Andere Bezeichnung: Sgo1

Hintergrund: SGOL1, SGO1, Shugoshin-like 1, hSgo1, Serologically defined breast cancer antigen NY-BR-85Shugoshin-like 1 encoded by SGO1 is a member of the shugoshin family of proteins. This protein is thought to protect centromeric cohesin from cleavage during mitotic prophase by preventing phosphorylation of a cohesin subunit. Reduced expression of this gene leads to the premature loss of centromeric cohesion, mis-segregation of sister chromatids, and mitotic arrest. Evidence suggests that this protein also protects a small subset of cohesin found along the length of the chromosome arms during mitotic prophase. An isoform lacking exon 6 has been shown to play a role in the cohesion of centrioles (PMID: 16582621 and PMID: 18331714). Mutations in this gene have been associated with Chronic Atrial and Intestinal Dysrhythmia (CAID) syndrome, characterized by the co-occurrence of Sick Sinus Syndrome (SSS) and Chronic Intestinal Pseudo-obstruction (CIPO) within the first four decades of life (PMID: 25282101). Fibroblast cells from CAID patients exhibited both increased cell proliferation and higher rates of senescence. Pseudogenes of this gene have been found on chromosomes 1 and 7. Alternative splicing results in multiple transcript variants.

Gen-ID: 151648

UniProt: Q5FBB7

Pathways: Maintenance of Protein Location