RFWD3 Antikörper (AA 350-430)

Produktnummer ABIN7223390

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch RFWD3 in WB, ELISA, IHC und IF. Es zeigt Reaktivität gegenüber Proben von Human und Maus.

Kurzübersicht für RFWD3 Antikörper (AA 350-430) (ABIN7223390)

Target: RFWD3 (Ring Finger and WD Repeat Domain 3 (RFWD3))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RFWD3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-RFWD3 Antikörper

Bindungsspezifität: AA 350-430

Verwendungszweck: Rabbit Anti-RFWD3 Polyclonal Antibody

Spezifität: RFWD3 Polyclonal Antibody detects endogenous levels of RFWD3 protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human RFWD3 at AA range: 350-430

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu RFWD3

Target: RFWD3 (Ring Finger and WD Repeat Domain 3 (RFWD3))

Andere Bezeichnung: RFWD3

Hintergrund: RFWD3, RNF201, E3 ubiquitin-protein ligase RFWD3, RING finger and WD repeat domain-containing protein 3, RING finger protein 201RFWD3, also named as RNF201, is a E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. RFWD3 may act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, It interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53. (PMID: 20173098 ) From BLAST result, this antibody is specific to RFWD3. 45kd and 55kd maybe splicesome of RFWD3.

Molekulargewicht: 85kD

Gen-ID: 55159

UniProt: Q6PCD5