RFWD3 Antikörper (AA 350-430)

Produktnummer ABIN7223390

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch RFWD3 in WB, ELISA und IHC (p). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für RFWD3 Antikörper (AA 350-430) (ABIN7223390)

Target: RFWD3 (Ring Finger and WD Repeat Domain 3 (RFWD3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RFWD3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-RFWD3 Antikörper

Bindungsspezifität: AA 350-430

Verwendungszweck: RFWD3 Polyclonal Antibody

Spezifität: RFWD3 Polyclonal Antibody detects endogenous levels of RFWD3 protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human RFWD3 at AA range: 350-430

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.

Kommentare:

Primary Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Details zu RFWD3

Target: RFWD3 (Ring Finger and WD Repeat Domain 3 (RFWD3))

Andere Bezeichnung: RFWD3

Hintergrund: Rabbit Anti-RFWD3 Polyclonal Antibody,RFWD3, RNF201, E3 ubiquitin-protein ligase RFWD3, RING finger and WD repeat domain-containing protein 3, RING finger protein 201,RFWD3, also named as RNF201, is a E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. RFWD3 may act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, It interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53. (PMID: 20173098 ) From BLAST result, this antibody is specific to RFWD3. 45kd and 55kd maybe splicesome of RFWD3.,E3 ubiquitin-protein ligase RFWD3

Molekulargewicht: observerd band 85kDa

Gen-ID: 55159

UniProt: Q6PCD5