Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:20000,Not yet tested in other applications.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C
Informationen zur Lagerung
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Haltbarkeit
12 months
Target
RIMKLA
(Ribosomal Modification Protein RimK-Like Family Member A (RIMKLA))
Andere Bezeichnung
FAM80A
Hintergrund
RIMKLA, FAM80A, N-acetylaspartyl-glutamate synthetase A, NAAG synthetase A, NAAGS, Ribosomal protein S6 modification-like protein ARIMKLA (N-acetylaspartyl-glutamate synthetase A) is a 391 amino acid protein that belongs to the rimK family. Localizing to cytoplasm, RIMKLA contains one ATP-grasp domain and binds two manganese ions per subunit. RIMKLA catalyzes the synthesis of N-acetylaspartyl-glutamate (NAAG). RIMKLA, is almost exclusively expressed in the CNS, whereas RIMKLB, which shares 65 % sequence identity with RIMKLA, is expressed in CNS and testis. It has also been suggested that RIMKLA and RIMKLB also served to ligate free glutamate to (an) acceptor (s). RIMKLA catalyzed the ATP-dependent synthesis of N-acetylaspartylglutamate (NAAG) from N-acetylaspartate and L-glutamate. RIMKLB catalyzed this reaction as well as the synthesis of β-citrylglutamate. The RIMKLA gene is conserved in chimpanzee, dog, cow and mouse, and maps to human chromosome 1p34.2.