PI3K p85 alpha/gamma Antikörper

Produktnummer ABIN7216546

Der Kaninchen Polyklonal anti-PI3K p85 alpha/gamma Antikörper (ABIN7216546) detektiert spezifisch PI3K p85 alpha/gamma in WB, ELISA, IF und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte, Affe und Huhn.

Kurzübersicht für PI3K p85 alpha/gamma Antikörper (ABIN7216546)

Target: PI3K p85 alpha/gamma (PI3K p85a/g) (Phosphoinositide 3 Kinase, p85 alpha/gamma (PI3K p85a/g))

Reaktivität: Human, Maus, Ratte, Affe, Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PI3K p85 alpha/gamma Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Produktdetails anti-PI3K p85 alpha/gamma Antikörper

Verwendungszweck: Rabbit Anti-PI 3-kinase p85α/γ Polyclonal Antibody

Spezifität: PI 3-kinase p85α/γ Polyclonal Antibody detects endogenous levels of PI 3-kinase p85α/γ protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human PI 3-kinase p85/p55 around the non-phosphorylation site of Y458/199

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu PI3K p85 alpha/gamma

Target: PI3K p85 alpha/gamma (PI3K p85a/g) (Phosphoinositide 3 Kinase, p85 alpha/gamma (PI3K p85a/g))

Andere Bezeichnung: PI 3-kinase p85alpha/gamma

Hintergrund: PIK3R1, GRB1, Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alphPhosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. PIK3R1 encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in PIK3R1 has been associated with insulin resistance. Alternative splicing of PIK3R1 results in four transcript variants encoding different isoforms.

Molekulargewicht: 54kD,83kD

Gen-ID: 5295, 8503

UniProt: P27986, Q92569