P35 (AA 30-110) Antikörper

Produktnummer ABIN7216376

Der Kaninchen Polyklonal anti-P35 Antikörper (ABIN7216376) detektiert spezifisch P35 in WB, ELISA, IF und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für P35 (AA 30-110) Antikörper (ABIN7216376)

Target: P35

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Produktdetails

Bindungsspezifität: AA 30-110

Verwendungszweck: Rabbit Anti-p35 Polyclonal Antibody

Spezifität: P35 Polyclonal Antibody detects endogenous levels of p35 protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human p35 at AA range: 30-110

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Antigendetails

Target: P35

Substanzklasse: Viral Protein

Hintergrund: CDK5R1, CDK5R, NCK5A, Cyclin-dependent kinase 5 activator 1, CDK5 activator 1, Cyclin-dependent kinase 5 regulatory subunit 1, TPKII regulatory subunitCyclin dependent kinase 5 regulatory subunit 1 encoded by CDK5R1 (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5), the activation of CDK5 is required for proper development of the central nervous system. The p35 form of cyclin dependent kinase 5 regulatory subunit 1 is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease.

Molekulargewicht: 38kD

Gen-ID: 8851

UniProt: Q15078