NFYB Antikörper (AA 10-90)

Produktnummer ABIN7216048

Der Kaninchen Polyklonal anti-NFYB Antikörper wird verwendet zum Nachweis von NFYB in Proben von Human, Maus und Ratte. Er wurde validiert für WB, ELISA, IHC und IF.

Kurzübersicht für NFYB Antikörper (AA 10-90) (ABIN7216048)

Target: NFYB (Nuclear Transcription Factor Y, beta (NFYB))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NFYB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-NFYB Antikörper

Bindungsspezifität: AA 10-90

Verwendungszweck: Rabbit Anti-NF-YB Polyclonal Antibody

Spezifität: NF-YB Polyclonal Antibody detects endogenous levels of NF-YB protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human NF-YB at AA range: 10-90

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu NFYB

Target: NFYB (Nuclear Transcription Factor Y, beta (NFYB))

Andere Bezeichnung: NF-YB

Hintergrund: NFYB, HAP3, Nuclear transcription factor Y subunit beta, CAAT box DNA-binding protein subunit B, Nuclear transcription factor Y subunit B, NF-YBNuclear transcription factor Y subunit beta encoded by NFYB is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. NFYB product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence, protein sequence alignments and experiments with mutants.

Molekulargewicht: 29kD

Gen-ID: 4801

UniProt: P25208

Pathways: Regulation of Lipid Metabolism by PPARalpha