NOTCH2 Antikörper (Cleaved-Asp1733)

Produktnummer ABIN7212500

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch NOTCH2 in WB, ELISA, IF und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für NOTCH2 Antikörper (Cleaved-Asp1733) (ABIN7212500)

Target: NOTCH2 (Notch 2 (NOTCH2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NOTCH2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-NOTCH2 Antikörper

Bindungsspezifität: AA 1660-1740, Cleaved-Asp1733

Verwendungszweck: Cleaved-Notch 2 (D1733) Polyclonal Antibody

Spezifität: Cleaved-Notch 2 (D1733) Polyclonal Antibody detects endogenous levels of fragment of activated Notch 2 protein resulting from cleavage adjacent to D1733.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human Notch 2 at AA range: 1660-1740

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.

Kommentare:

Primary Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Details zu NOTCH2

Target: NOTCH2 (Notch 2 (NOTCH2))

Andere Bezeichnung: Notch 2

Hintergrund: Rabbit Anti-Cleaved-Notch 2 (D1733) Polyclonal Antibody,NOTCH2, Neurogenic locus notch homolog protein 2, Notch 2, hN2,NOTCH2 encodes a member of the NOTCH family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands(delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. Notch 2 is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. Notch 2 functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. Two transcript variants encoding different isoforms have been found for NOTCH2.,Neurogenic locus notch homolog protein 2

Molekulargewicht: observerd band 100+130kDa

Gen-ID: 4853

UniProt: Q04721

Pathways: Notch Signalweg, Stem Cell Maintenance