NCF4 Antikörper (pThr154)

Produktnummer ABIN7222664

Der Kaninchen Polyklonal anti-NCF4 Antikörper (ABIN7222664) detektiert spezifisch NCF4 in ELISA und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für NCF4 Antikörper (pThr154) (ABIN7222664)

Target: NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NCF4 Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-NCF4 Antikörper

Bindungsspezifität: pThr154

Verwendungszweck: p40-phox (phospho Thr154) Polyclonal Antibody

Spezifität: Phospho-p40-phox (T154) Polyclonal Antibody detects endogenous levels of p40-phox protein only when phosphorylated at T154.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human p40-phox Phospho-Thr154

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.

Kommentare:

Primary Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Details zu NCF4

Target: NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))

Andere Bezeichnung: p40-phox

Hintergrund: Rabbit Anti-p40-phox (phospho Thr154) Polyclonal Antibody,NCF4, SH3PXD4, Neutrophil cytosol factor 4, NCF-4, Neutrophil NADPH oxidase factor 4, SH3 and PX domain-containing protein 4, p40-phox, p40phox,The protein encoded by NCF4 is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI (3) kinase, which suggests its role in PI (3) kinase-mediated signaling events. The phosphorylation of neutrophil cytosolic factor 4 was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.,Neutrophil cytosol factor 4

Gen-ID: 4689

UniProt: Q15080