MYL12B Antikörper (pSer18)

Produktnummer ABIN7221772

Der Kaninchen Polyklonal Anti-MYL12B Antikörper wurde für WB, ELISA, IF und IHC (p) validiert. Er ist geeignet, MYL12B in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für MYL12B Antikörper (pSer18) (ABIN7221772)

Target: MYL12B (Myosin, Light Chain 12B, Regulatory (MYL12B))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MYL12B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-MYL12B Antikörper

Bindungsspezifität: pSer18

Verwendungszweck: MRLC2 (phospho Ser18) Polyclonal Antibody

Spezifität: Phospho-MRLC2 (S18) Polyclonal Antibody detects endogenous levels of MRLC2 protein only when phosphorylated at S18.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human MRLC2 Phospho-Ser18

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.

Kommentare:

Primary Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Details zu MYL12B

Target: MYL12B (Myosin, Light Chain 12B, Regulatory (MYL12B))

Andere Bezeichnung: MRLC2

Hintergrund: Rabbit Anti-MRLC2 (phospho Ser18) Polyclonal Antibody,MYL9, MLC2, MRLC1, MYRL2, Myosin regulatory light polypeptide 9, 20 kDa myosin light chain, LC20, MLC-2C, Myosin RLC, Myosin regulatory light chain 2, smooth muscle isoform, Myosin regulatory light chain 9, Myosin regulatory light chain MRL,Myosin is a highly conserved, ubiquitously expressed protein that interacts with Actin to generate the force for cellular movements. Conventional myosins are hexameric proteins consisting of two heavy chain subunits, a pair of non-phosphorylatable light chain subunits and a pair of phosphorylatable light chain subunits. Three general classes of myosin have been cloned: smooth muscle myosins, striated muscle myosins and non-muscle myosins. Myosin regulatory light chains, including MYL12A (also known as MRLC3 or MLCB), MYL12B (also known as MRLC2) and MYL9 (also known as LC20, MLC2, MRLC1 or MYRL2), regulate contraction in smooth muscle and non-muscle cells via phosphorylation by myosin light chain kinase (MLCK). Phosphorylation of myosin regulatory light chains, catalyzed by MLCK in the presence of calcium and calmodulin, increases the Actin-activated myosin ATPase activity, thereby regulating the contractile activity. Myosin light chain is also located in striated skeletal muscle, where its function remains undefined.,Myosin regulatory light polypeptide 9

Molekulargewicht: observerd band 18kDa

Gen-ID: 3566

UniProt: P24844

Pathways: Feeding Behaviour