MAPK8IP1 Antikörper (pThr103)

Produktnummer ABIN7225994

Der Kaninchen Polyklonal anti-MAPK8IP1 Antikörper wird verwendet zum Nachweis von MAPK8IP1 in Proben von Human, Ratte und Maus. Er wurde validiert für WB, ELISA, IF und IHC.

Kurzübersicht für MAPK8IP1 Antikörper (pThr103) (ABIN7225994)

Target: MAPK8IP1 (Mitogen-Activated Protein Kinase 8 Interacting Protein 1 (MAPK8IP1))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MAPK8IP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Produktdetails anti-MAPK8IP1 Antikörper

Bindungsspezifität: pThr103

Verwendungszweck: Rabbit Anti-JIP-1 (phospho Thr103) Polyclonal Antibody

Spezifität: Phospho-JIP-1 (T103) Polyclonal Antibody detects endogenous levels of JIP-1 protein only when phosphorylated at T103.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human JIP-1 Phospho-Thr103

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:5000,Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu MAPK8IP1

Target: MAPK8IP1 (Mitogen-Activated Protein Kinase 8 Interacting Protein 1 (MAPK8IP1))

Andere Bezeichnung: JIP-1

Hintergrund: MAPK8IP1, IB1, JIP1, PRKM8IP, C-Jun-amino-terminal kinase-interacting protein 1, JIP-1, JNK-interacting protein 1, Islet-brain 1, IB-1, JNK MAP kinase scaffold protein 1, Mitogen-activated protein kinase 8-interacting protein 1MAPK8IP1 (mitogen-activated protein kinase 8 interacting protein 1) encodes a regulator of the pancreatic beta-cell function. It is highly similar to JIP-1, a mouse protein known to be a regulator of c-Jun amino-terminal kinase (Mapk8). This protein has been shown to prevent MAPK8 mediated activation of transcription factors, and to decrease IL-1 beta and MAP kinase kinase 1 (MEKK1) induced apoptosis in pancreatic beta cells. This protein also functions as a DNA-binding transactivator of the glucose transporter GLUT2. RE1-silencing transcription factor (REST) is reported to repress the expression of this gene in insulin-secreting beta cells. MAPK8IP1 is found to be mutated in a type 2 diabetes family, and thus is thought to be a susceptibility gene for type 2 diabetes.

Molekulargewicht: 113kD

Gen-ID: 9479

UniProt: Q9UQF2