MICU1 Antikörper

Produktnummer ABIN7206382

Der Maus Monoklonal anti-MICU1 Antikörper (ABIN7206382) detektiert spezifisch MICU1 in WB, IHC und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte und Maus.

Kurzübersicht für MICU1 Antikörper (ABIN7206382)

Target: MICU1 (Mitochondrial Calcium Uptake 1 (MICU1))

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MICU1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-MICU1 Antikörper

Verwendungszweck: Mouse Anti-MICU1 Monoclonal Antibody

Spezifität: The antibody detects endogenous MICU1 protein.

Aufreinigung: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

Immunogen: Recombinant Protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:1000-2000,IHC 1:100-200,IF 1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.5 % BSA, 0.02 % sodium azide as Preservative and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu MICU1

Target: MICU1 (Mitochondrial Calcium Uptake 1 (MICU1))

Andere Bezeichnung: MICU1

Hintergrund: Most PKMT substrates are histone proteins and transcription factors, emphasizing the importance of lysine methylation in regulating chromatin structure and gene expression. Lys9 of histone H3 is mono- or di-methylated by G9A/GLP and tri-methylated by SETDB1 to activate transcription. JHDM3A-mediated demethylation of the same residue creates mono-methyl Lys9 and inhibits gene transcription . Tumor suppressor p53 is regulated by methylation of at least four sites. p53-mediated transcription is repressed following mono-methylation of p53 at Lys370 by SMYD2, di-methylation at the same residue further inhibits p53 by preventing association with 53BP1. Concomitant di-methylation at Lys382 inhibits p53 ubiquitination following DNA damage. Mono-methylation at Lys382 by SET8 suppresses p53 transcriptional activity, while SET7/9 mono-methylation at Lys372 inhibits SMYD2 methylation at Lys370 and stabilizes the p53 protein. Di-methylation at Lys373 by G9A/GLP inhibits p53-mediated apoptosis and correlates with tri-methylation of histone H3 Lys9 at the p21 promoter . Overexpression of PKMTs is associated with multiple forms of human cancer, which has generated tremendous interest in targeting protein lysine methyltransferases in drug discovery research.

Molekulargewicht: 55kD

Gen-ID: 10367

UniProt: Q9BPX6