MED23 Antikörper (AA 10-90)

Produktnummer ABIN7214346

Der Kaninchen Polyklonal anti-MED23 Antikörper wird verwendet zum Nachweis von MED23 in Proben von Human, Ratte und Maus. Er wurde validiert für ELISA, WB, IHC und IF.

Kurzübersicht für MED23 Antikörper (AA 10-90) (ABIN7214346)

Target: MED23 (Mediator Complex Subunit 23 (MED23))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MED23 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-MED23 Antikörper

Bindungsspezifität: AA 10-90

Verwendungszweck: Rabbit Anti-CRSP130 Polyclonal Antibody

Spezifität: CRSP130 Polyclonal Antibody detects endogenous levels of CRSP130 protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human CRSP130 at AA range: 10-90

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:5000,Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu MED23

Target: MED23 (Mediator Complex Subunit 23 (MED23))

Andere Bezeichnung: CRSP130

Hintergrund: MED23, ARC130, CRSP3, DRIP130, KIAA1216, SUR2, Mediator of RNA polymerase II transcription subunit 23, Activator-recruited cofactor 130 kDa component, ARC130, Cofactor required for Sp1 transcriptional activation subunit 3, CRSP complex subuThe activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. Mediator complex subunit 23 encoded by MED23 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein also acts as a metastasis suppressor. Several alternatively spliced transcript variants encoding different isoforms have been described for MED23.

Molekulargewicht: 150kD

Gen-ID: 9439

UniProt: Q9ULK4

Pathways: Regulation of Lipid Metabolism by PPARalpha