MDH1 Antikörper

Produktnummer ABIN7229614

Der Kaninchen Polyklonal anti-MDH1 Antikörper (ABIN7229614) detektiert spezifisch MDH1 in WB und ELISA. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für MDH1 Antikörper (ABIN7229614)

Target: MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MDH1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-MDH1 Antikörper

Verwendungszweck: Rabbit Anti-MDHC Polyclonal Antibody

Spezifität: The antibody detects endogenous levels of MDHC protein

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human MDHC protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu MDH1

Target: MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

Andere Bezeichnung: MDHC

Hintergrund: Malate dehydrogenase, cytoplasmic, Cytosolic malate dehydrogenase, Diiodophenylpyruvate reductaseMDH1 encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. Alternatively spliced transcript variants have been found for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 6.

Molekulargewicht: 36kD

Gen-ID: 4190

UniProt: P40925