LIMK2 Antikörper (pSer283)

Produktnummer ABIN7222220

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch LIMK2 in WB, ELISA und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte und Affe.

Kurzübersicht für LIMK2 Antikörper (pSer283) (ABIN7222220)

Target: LIMK2 (LIM Domain Kinase 2 (LIMK2))

Reaktivität: Human, Maus, Ratte, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LIMK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-LIMK2 Antikörper

Bindungsspezifität: pSer283

Verwendungszweck: LIMK-2 (phospho Ser283) Polyclonal Antibody

Spezifität: Phospho-LIMK-2 (S283) Polyclonal Antibody detects endogenous levels of LIMK-2 protein only when phosphorylated at S283.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human LIMK-2 Phospho-Ser283

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.

Kommentare:

Primary Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Details zu LIMK2

Target: LIMK2 (LIM Domain Kinase 2 (LIMK2))

Andere Bezeichnung: LIMK-2

Hintergrund: Rabbit Anti-LIMK-2 (phospho Ser283) Polyclonal Antibody,LIMK2, LIM domain kinase 2, LIMK-2,There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by LIMK2 is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene.,LIM domain kinase 2

Molekulargewicht: observerd band 72kDa

Gen-ID: 3985

UniProt: P53671