Der Kaninchen Polyklonal anti-ICK Antikörper wird verwendet zum Nachweis von ICK in Proben von Human, Maus und Ratte. Er wurde validiert für WB, ELISA und IHC.
Phospho-Intestinal Cell Kinase (Y159) Polyclonal Antibody detects endogenous levels of Intestinal Cell Kinase protein only when phosphorylated at Y159.
Aufreinigung
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Immunogen
Synthesized peptide derived from human Intestinal Cell Kinase Phospho-Tyr159
ICK
Reaktivität: Human
WB
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-300,ELISA 1:2000-20000
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C
Informationen zur Lagerung
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Haltbarkeit
12 months
Target
ICK
(Intestinal Cell (MAK-Like) Kinase (ICK))
Andere Bezeichnung
Intestinal Cell Kinase
Hintergrund
ICK, KIAA0936, Serine/threonine-protein kinase ICK, Intestinal cell kinase, hICK, Laryngeal cancer kinase 2, LCK2, MAK-related kinase, MRKEukaryotic protein kinases are enzymes that belong to a very extensive family of proteins which share a conserved catalytic core common with both serine/threonine and tyrosine protein kinases. ICK encodes an intestinal serine/threonine kinase harboring a dual phosphorylation site found in mitogen-activating protein (MAP) kinases. The intestinal cell kinase localizes to the intestinal crypt region and is thought to be important in intestinal epithelial cell proliferation and differentiation. Alternative splicing has been observed at this locus and two variants, encoding the same isoform, have been identified.