Der Maus Monoklonal Anti-Histone H2B Antikörper wurde für WB und IF validiert. Er ist geeignet, Histone H2B in Proben von Human, Maus und Ratte zu detektieren.
Reaktivität: Human
WB, ELISA, IF, ChIP
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:3000), IF (1:100-1:200).
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
PBS, pH 7.4, containing 0.02 % Sodium Azide as preservative and 50 % Glycerol.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C
Informationen zur Lagerung
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Target
Histone H2B
Hintergrund
Mouse Anti-Histone H2B Monoclonal Antibody,HIST1H2BB, H2BFF, Histone H2B type 1-B, Histone H2B.1, Histone H2B.f, H2B/f,Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. HIST1H2BB is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from HIST1H2BB lack polyA tails, instead, they contain a palindromic termination element. HIST1H2BB is found in the large histone gene cluster on chromosome 6p22-p21.3.,Histone H2B type 1-B