His Tag Antikörper

Produktnummer ABIN7212544

Der Kaninchen Polyklonal anti-His Tag Antikörper (ABIN7212544) detektiert spezifisch His Tag in WB und ELISA.

Kurzübersicht für His Tag Antikörper (ABIN7212544)

Target: His Tag

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser His Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-His Tag Antikörper

Verwendungszweck: Rabbit Anti-His-tag Polyclonal Antibody

Spezifität: His-tag Polyclonal Antibody detects His-tagged recombinant proteins or His-tagged proteins overexpressed in cells.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: 6X His synthetic peptide conjugated to KLH

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:1000-1:3000,ELISA 1:20000,Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu His Tag

Target: His Tag

Andere Bezeichnung: His-tag

Substanzklasse: Tag

Hintergrund: His, 6 His epitope, Hexa His, HHHHHH epitope, Polyhistidine TagThe his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein's structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.

Molekulargewicht: 15kD