GAD65+GAD67 (AA 520-600) Antikörper

Produktnummer ABIN7214988

Der Kaninchen Polyklonal anti-GAD65+GAD67 Antikörper (ABIN7214988) detektiert spezifisch GAD65+GAD67 in WB, IHC, ELISA und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Maus und Human.

Kurzübersicht für GAD65+GAD67 (AA 520-600) Antikörper (ABIN7214988)

Target: GAD65+GAD67

Reaktivität: Maus, Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Produktdetails

Bindungsspezifität: AA 520-600

Verwendungszweck: Rabbit Anti-GAD-65/67 Polyclonal Antibody

Spezifität: GAD-65/67 Polyclonal Antibody detects endogenous levels of GAD-65/67 protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human GAD-65/67 at AA range: 520-600

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:5000,Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Antigendetails

Target: GAD65+GAD67

Andere Bezeichnung: GAD-65/67

Hintergrund: GAD1, GAD, GAD67, Glutamate decarboxylase 1, 67 kDa glutamic acid decarboxylase, GAD-67, Glutamate decarboxylase 67 kDa isoform, GAD2, GAD65, Glutamate decarboxylase 2, 65 kDa glutamic acid decarboxylase, GAD-65, Glutamate decarboxylase 65GAD1 encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. Glutamate decarboxylase 1 is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.

Molekulargewicht: 65kD

Gen-ID: 2571, 2572

UniProt: Q99259, Q05329