EIF5B Antikörper (AA 1020-1100)

Produktnummer ABIN7226064

Der Kaninchen Polyklonal anti-EIF5B Antikörper (ABIN7226064) detektiert spezifisch EIF5B in WB, ELISA, IF und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für EIF5B Antikörper (AA 1020-1100) (ABIN7226064)

Target: EIF5B (Eukaryotic Translation Initiation Factor 5B (EIF5B))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EIF5B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Produktdetails anti-EIF5B Antikörper

Bindungsspezifität: AA 1020-1100

Verwendungszweck: Rabbit Anti-eIF5B Polyclonal Antibody

Spezifität: EIF5B Polyclonal Antibody detects endogenous levels of eIF5B protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human eIF5B at AA range: 1020-1100

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu EIF5B

Target: EIF5B (Eukaryotic Translation Initiation Factor 5B (EIF5B))

Andere Bezeichnung: eIF5B

Hintergrund: EIF5B, IF2, KIAA0741, Eukaryotic translation initiation factor 5B, eIF-5B, Translation initiation factor IF-2Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B (eukaryotic translation initiation factor 5B). The gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately.

Molekulargewicht: 139kD

Gen-ID: 9669

UniProt: O60841