EIF4G2 Antikörper (AA 10-90)

Produktnummer ABIN7214510

Der Kaninchen Polyklonal anti-EIF4G2 Antikörper wird verwendet zum Nachweis von EIF4G2 in Proben von Human und Maus. Er wurde validiert für WB, ELISA, IHC und IF.

Kurzübersicht für EIF4G2 Antikörper (AA 10-90) (ABIN7214510)

Target: EIF4G2 (Eukaryotic Translation Initiation Factor 4 gamma 2 (EIF4G2))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EIF4G2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-EIF4G2 Antikörper

Bindungsspezifität: AA 10-90

Verwendungszweck: Rabbit Anti-DAP-5 Polyclonal Antibody

Spezifität: DAP-5 Polyclonal Antibody detects endogenous levels of DAP-5 protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human DAP-5 at AA range: 10-90

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu EIF4G2

Target: EIF4G2 (Eukaryotic Translation Initiation Factor 4 gamma 2 (EIF4G2))

Andere Bezeichnung: DAP-5

Hintergrund: EIF4G2, DAP5, OK/SW-cl.75, Eukaryotic translation initiation factor 4 gamma 2, eIF-4-gamma 2, eIF-4G 2, eIF4G 2, Death-associated protein 5, DAP-5, p97Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. Eukaryotic translation initiation factor 4 gamma 2 encoded by EIF4G2 shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3, eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, EIF4G2 product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of EIF4G2 have been described.

Molekulargewicht: 90kD

Gen-ID: 1982

UniProt: P78344