E2F4/E2F5 (E2F4/5) (AA 30-110) Antikörper

Produktnummer ABIN7214648

Dieser Anti- Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von in WB, ELISA und IHC (p). Geeignet für Human, Maus, Ratte und Affe.

Kurzübersicht für E2F4/E2F5 (E2F4/5) (AA 30-110) Antikörper (ABIN7214648)

Target: E2F4/E2F5 (E2F4/5)

Reaktivität: Human, Maus, Ratte, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails

Bindungsspezifität: AA 30-110

Verwendungszweck: E2F-4/5 Polyclonal Antibody

Spezifität: E2F-4/5 Polyclonal Antibody detects endogenous levels of E2F-4/5 protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human E2F-4/5 at AA range: 30-110

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.

Kommentare:

Primary Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Antigendetails

Target: E2F4/E2F5 (E2F4/5)

Andere Bezeichnung: E2F-4/5

Hintergrund: Rabbit Anti-E2F-4/5 Polyclonal Antibody,E2F4, Transcription factor E2F4, E2F-4, E2F5, Transcription factor E2F5, E2F-5,E2F transcription factor 4 encoded by E2F4 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer.,Transcription factor E2F4

Molekulargewicht: observerd band 44kDa

Gen-ID: 1874, 1875

Pathways: Mitotic G1-G1/S Phases