DOK3 Antikörper (AA 70-150)

Produktnummer ABIN7214606

Der Kaninchen Polyklonal anti-DOK3 Antikörper wird verwendet zum Nachweis von DOK3 in Proben von Human und Maus. Er wurde validiert für WB, ELISA, IHC und IF.

Kurzübersicht für DOK3 Antikörper (AA 70-150) (ABIN7214606)

Target: DOK3 (Docking Protein 3 (DOK3))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DOK3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-DOK3 Antikörper

Bindungsspezifität: AA 70-150

Verwendungszweck: Rabbit Anti-Dok-3 Polyclonal Antibody

Spezifität: Dok-3 Polyclonal Antibody detects endogenous levels of Dok-3 protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Dok-3 at AA range: 70-150

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu DOK3

Target: DOK3 (Docking Protein 3 (DOK3))

Andere Bezeichnung: Dok-3

Hintergrund: DOK3, Docking protein 3, Downstream of tyrosine kinase 3Dok-1, Dok-2 and Dok-3 are members of a class of "docking" proteins which contain multiple tyrosine residues and putative SH2 binding sites. Dok-1 associates with the Ras GTPase activating protein (Ras GAP) upon tyrosine phosphorylation. Dok-2 (also designated p56 Dok) has also been identified as a potential mediator of the effects of p210 Bcr-Abl. Dok-3 is an adapter involved in the recruitment of inhibitory molecules and is highly expressed in B cells and macrophages. Immunoreceptor-mediated cellular activation induces tyrosine phosphorylation of Dok-3. Upon phosphorylation, Dok-3 binds to 5' inositol phosphatase SHIP and the protein tyrosine kinase Csk. Dok-3 may play a significant role in the negative regulation of immunoreceptor signaling in hemopoietic cells.

Molekulargewicht: 58kD

Gen-ID: 79930

UniProt: Q7L591