DNA Polymerase I (AA 610-690) Antikörper

Produktnummer ABIN7219268

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch not_set in ELISA und WB. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für DNA Polymerase I (AA 610-690) Antikörper (ABIN7219268)

Target: DNA Polymerase I

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: ELISA, Western Blotting (WB)

Produktdetails

Bindungsspezifität: AA 610-690

Verwendungszweck: DNA pol ι Polyclonal Antibody

Spezifität: DNA pol ι Polyclonal Antibody detects endogenous levels of DNA pol ι protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human DNA pol ι at AA range: 610-690

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:40000). Not yet tested in other applications.

Kommentare:

Primary Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Antigendetails

Target: DNA Polymerase I

Andere Bezeichnung: DNA pol ι

Hintergrund: Rabbit Anti-DNA pol ι Polyclonal Antibody,POLI, RAD30B, DNA polymerase iota, Eta2, RAD30 homolog B,POLI (Polymerase (DNA) Iota) is a Protein Coding gene. Among its related pathways are DNA Double-Strand Break Repair and Fanconi anemia pathway. GO annotations related to this gene include damaged DNA binding and DNA-directed DNA polymerase activity. An important paralog of this gene is POLH. rror-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5-deoxyribose phosphate at abasic sites, but may not have lyase activity.,DNA polymerase iota

Molekulargewicht: observerd band 85kDa

Gen-ID: 11201

UniProt: Q9UNA4