Complement B + Bb (AA 210-290), (Cleaved-Lys260) Antikörper

Produktnummer ABIN7224154

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Complement B + Bb in WB und ELISA. Es zeigt Reaktivität gegenüber Proben von Human und Maus.

Kurzübersicht für Complement B + Bb (AA 210-290), (Cleaved-Lys260) Antikörper (ABIN7224154)

Target: Complement B + Bb

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails

Bindungsspezifität: AA 210-290, Cleaved-Lys260

Verwendungszweck: Rabbit Anti-Cleaved-Factor B Bb (K260) Polyclonal Antibody

Spezifität: Cleaved-Factor B Bb (K260) Polyclonal Antibody detects endogenous levels of fragment of activated Factor B Bb protein resulting from cleavage adjacent to K260.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Factor B Bb at AA range: 210-290

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:20000,Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Antigendetails

Target: Complement B + Bb

Andere Bezeichnung: Factor B Bb

Hintergrund: CFB, BF, BFD, Complement factor B, C3/C5 convertase, Glycine-rich beta glycoprotein, GBG, PBF2, Properdin factor BCFB (complement factor B) encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. CFB localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. Polymorphisms in CFB are associated with a reduced risk of age-related macular degeneration. The polyadenylation site of CFB is 421 bp from the 5' end of the gene for complement component 2.

Molekulargewicht: 57kD,85kD

Gen-ID: 629

UniProt: P00751