Cofilin Antikörper (pSer3)

Produktnummer ABIN7221928

Dieser Anti-Cofilin Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von Cofilin in WB, ELISA und IHC (p). Geeignet für Human, Maus und Ratte.

Kurzübersicht für Cofilin Antikörper (pSer3) (ABIN7221928)

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cofilin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Cofilin Antikörper

Bindungsspezifität: pSer3

Verwendungszweck: Cofilin (phospho Ser3) Polyclonal Antibody

Spezifität: Phospho-Cofilin (S3) Polyclonal Antibody detects endogenous levels of Cofilin protein only when phosphorylated at S3.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Cofilin Phospho-Ser3

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Kommentare:

Primary Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Details zu Cofilin

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Andere Bezeichnung: Cofilin

Hintergrund: Rabbit Anti-Cofilin (phospho Ser3) Polyclonal Antibody,CFL1, CFL, Cofilin-1, 18 kDa phosphoprotein, p18, Cofilin, non-muscle isoform,Cofilin is ubiquitously expressed in eukaryotic cells where it binds to actin, thereby regulating the rapid cycling of actin assembly and disassembly essential for cellular viability. Cofilin is a low molecular weight protein that binds to filamentous (F) actin by bridging two longitudinally associated actin subunits changing the F-actin filament twist. This process is allowed by the dephosphorylation of cofilin ser-3 by factors such as opsonized zymosan. Lim kinase 1, a serine kinase, phosphorylates cofilin and renders it unable to bind and depolymerise F-actin.,Cofilin-1

Molekulargewicht: observerd band 19kDa

Gen-ID: 3716

UniProt: P23528

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events