CDCA3 Antikörper (AA 190-270)

Produktnummer ABIN7214118

Dieser Anti-CDCA3 Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von CDCA3 in WB, ELISA, IF und IHC (p). Geeignet für Human, Ratte und Maus.

Kurzübersicht für CDCA3 Antikörper (AA 190-270) (ABIN7214118)

Target: CDCA3 (Cell Division Cycle Associated 3 (CDCA3))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDCA3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-CDCA3 Antikörper

Bindungsspezifität: AA 190-270

Verwendungszweck: CdcA3 Polyclonal Antibody

Spezifität: CdcA3 Polyclonal Antibody detects endogenous levels of CdcA3 protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human CdcA3 at AA range: 190-270

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.

Kommentare:

Primary Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Details zu CDCA3

Target: CDCA3 (Cell Division Cycle Associated 3 (CDCA3))

Andere Bezeichnung: CdcA3

Hintergrund: Rabbit Anti-CdcA3 Polyclonal Antibody,CDCA3, C8, GRCC8, TOME1, Cell division cycle-associated protein 3, Gene-rich cluster protein C8, Trigger of mitotic entry protein 1, TOME-1,Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division control (Cdc) genes are required at various points in the cell cycle. CdcA3 (Cell division cycle-associated protein 3), also known as TOME1 (Trigger of mitotic entry protein 1) and GRCC8 (Gene-rich cluster protein C8), is a 268 amino acid cytoplasmic protein that is required for entry into mitosis. By participating with E3 ligase complexes, CdcA3 is involved in ubiquination and degradation of the mitosis-inhibitory kinase Wee 1, leading to the dephosphorylation of Cdc2 in the late G2 phase. CdcA3 itself is targeted for destruction by anaphase promoting complex (APC) in the G1 phase of the cell cycle, then allowing for Wee 1 accumulation during interphase.,Cell division cycle-associated protein 3

Molekulargewicht: observerd band 28kDa

Gen-ID: 83461

UniProt: Q99618